Resveratrol prevents osteoporosis in ovariectomized rats by regulating microRNA-338-3p.
نویسندگان
چکیده
Osteoporosis is a disease characterized by loss of bone mass and degeneration of the microstructure of bone. Resveratrol (3,5,4-tri-hydroxystilbene; RESV) may delay the onset of a variety of age-related diseases. In the present study, an ovariectomized female rat model was used to detect the changes in microRNAs (miRNAs/miRs) following RESV treatment. Subsequently, the target genes of miRNA were predicted using TargetScan software and determined using a dual-luciferase reporter assay. Finally, the role of miR-338-3p in the proliferation and differentiation of human osteoblast (HOB) cells was confirmed. The predominant finding of the present study was the identification of an intact mechanism of the effect of RESV in osteoporosis treatment. The results suggested that RESV suppresses miR-338-3p, followed by an increase in the expression of runt-related transcription factor 2 in HOB cells.
منابع مشابه
Propranolol Prevents Osteoporosis and up-regulates Leptin in Ovariectomized Rats
Osteoporosis is a systemic skeletal disease and there is a close relationship between the sympathetic nervous system (SNS) and bone metabolism. Leptin has been shown to regulate bone formation and bone resorption via the SNS. However, the effect of SNS on leptin signaling has not been clearly understood. In the present study, we studied the effect of propranolol on ovariectomy-induced osteoporo...
متن کاملMicroRNA-338-3p inhibits glucocorticoid-induced osteoclast formation through RANKL targeting.
The differentiation deficiencies of osteoclast precursors (pre-OCs) may contribute to osteoporosis. Research on osteoporosis has recently focused on microRNAs (miRNAs) that play crucial roles in pre-OC differentiation. In the current study, we aimed to analyze the expression and function of the glucocorticoid (GC)-associated miRNA-338-3p (miR-338-3p) in osteoclast formation. We found that dexam...
متن کاملP-236: The Role of Trace Elements in Treatment of Ovariectomized Osteoporotic Rats
Background: Osteoporosis is one of the uncomfortable postmenopausal symptoms. The risk of imbalanced nutrition especially traces elements and vitamins are high during post menopause and may lead to osteoporosis due to reduction of content of mineral bone. The aim of this study was to investigate the potential consequence of Selenium (Se) treatment in ovariectomized rat model with osteoporosis i...
متن کاملPropranolol Prevents Osteoporosis and up-regulates Leptin in Ovariectomized Rats
Osteoporosis is a systemic skeletal disease and there is a close relationship between the sympathetic nervous system (SNS) and bone metabolism. Leptin has been shown to regulate bone formation and bone resorption via the SNS. However, the effect of SNS on leptin signaling has not been clearly understood. In the present study, we studied the effect of propranolol on ovariectomy-induced osteoporo...
متن کاملmiR-338-3p Is Down-Regulated by Hepatitis B Virus X and Inhibits Cell Proliferation by Targeting the 3′-UTR Region of CyclinD1
Hepatitis B virus X protein (HBx) is recognized as an oncogene in hepatocellular carcinoma (HCC). HBx regulates microRNA expression, including down-regulating miR-338-3p in LO2 cells. Here, we investigated miR-338-3p function in HBx-mediated hepatocarcinogenesis. In 23 HBV-infected HCC clinical patient tumor and adjacent non-tumor control tissues, 17 and 19 tumors expressed HBx mRNA and protein...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Molecular medicine reports
دوره 12 2 شماره
صفحات -
تاریخ انتشار 2015